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مقاله
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Abstract
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Title:
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Preparation, Characterization, and In Vivo Evaluation of Solid Lipid Nanoparticles Containing Diclofenac after Intravitreal Administration
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Author(s):
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Majid Abrishami , Bijan Malaekeh-Nikouei , Mojtaba Abrishami
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Presentation Type:
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Oral
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Subject:
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Posterior Segment and Uveitis
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Others:
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Presenting Author:
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Name:
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Mojtaba Abrishami
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Affiliation :(optional)
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Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran ; Eye Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
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E mail:
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mojtaba_abrishami@yahoo.com
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Phone:
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05138433192
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Mobile:
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09129377278
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Purpose:
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For improvement of drug availability after intravitreal administration, Solid Lipid Nanoparticles (SLNs) containing Diclofenac as a novel drug delivery system was prepared and compared with conventional formulas in experimental model after intravitreal injection.
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Methods:
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In this experimental clinical trial, 18 albino rabbits were included in the study. In right eyes of all rabbits SLNs of Diclofenac (0.3 mg) and in the left eyes of the rabbits, commercial form of Diclofenac (0.3 mg) was injected. One, four, twelve, twenty-four and forty-eight hours after injection, vitreous and aqueous humor samples were obtained. Concentration of diclofenac sodium was evaluated using HPLC method.
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Results:
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Size of nanoparticles was around 170 nm after preparation. Drug concentration in eyes injected by SLNs was significantly higher than left eyes injected by commercial product (p<0.001). Diclofenac was quantified in samples up to 48 hours after intraocular injection. Four hours after intravitreal injection, concentration of diclofenac in vitreous and aqueous humor of eyes received SLNs was respectively 2.5 and 6.5 times higher than eyes injected by commercial form of drug.
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Conclusion:
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SLN form of Diclofenac is beneficial in intravitreal durability of the drug. Moreover, although the drug was injected intravitreally, it has been found in the aqueous humor in a significant concentration.
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Attachment:
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