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مقاله
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Abstract
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Title:
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Determination of the safe dose of intravitreal minocycline in rabbit eyes
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Author(s):
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Khalil Ghasemi Falavarjani MD, Arash Pourhabibi MD, Kaveh Abri Aghdam MD, Hossein Nazari Khanamiri MD, Bagher Hosseini MD, Mehdi Modarres MD, Abdolreza Pazouki MD.
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Presentation Type:
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Oral
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Subject:
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Posterior Segment and Uveitis
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Others:
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Presenting Author:
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Name:
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Arash Pourhabibi
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Affiliation :(optional)
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Eye research Center, Rassoul Akram Hospital, Iran University of Medical Sciences
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E mail:
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dr.apourhabibi@yahoo.com
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Phone:
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5553349
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Mobile:
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09111373329
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Purpose:
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To evaluate the retinal toxicity of intravitreal minocycline in rabbit eye.
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Methods:
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Ten New Zealand albino rabbits, weighing between 2 and 3 kg were used for this study. The initial concentration of minocycline (25 mg) was titrated to obtain different concentrations of 1000 μg, 500 μg, 250 μg, 125 μg, and 62.5 μg in 0.1ml. Each concentration was injected into 2 rabbit eyes. For each dose, normal saline was injected in one contralateral eye and the other fellow eye remained non-injected. Electrophysiologic testing was performed before and 4 weeks after injections. The eyes were enucleated 4 weeks after injections and examined using light microscopy.
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Results:
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The clinical examination was unremarkable after injections. Electroretinography recordings were significantly affected at all doses in at least one of the a or b wave of cone, rod or maximal combined responses. Histopathologic examination revealed severe atrophy, disorganization, and loss of integrity in all retinal layers in all minocycline injected eyes. Contralateral eyes were normal.
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Conclusion:
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In our study, intravitreal minocycline was toxic to the retina in albino rabbits even at a concentration of 62.5µg/ml.
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Attachment:
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