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       XXV Annual Congress of the Iranian Society of Ophthalmology        بـیــست و پنجمین کنــگــره سـالیـانه انـجـمـن چـشـم پـزشـکی ایـــران
مقاله Abstract


Title: Cellular Response of Limbal Stem Cells on PHBV/Gelatin Nanofibers Scaffolds for Ocular Epithelial Regeneration
Author(s): Alireza Baradaran-Rafii , Seyed Hashem Daryabari , Esmaeil Biazar , Saeed Heidari-keshel
Presentation Type: Oral
Subject: Cornea and Anterior Segment
Others:
Presenting Author:
Name: Seyed hashem Daryabari
Affiliation :(optional) Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences
E mail: shdarya50@yahoo.com
Phone:
Mobile: 09113201959
Purpose:

The aim of this study was to develop a blend of nanofibrous Poly (3-hydroxybutyrate-co-3- hydroxyvalerate) (PHBV) / gelatin substrate for limbal stem cell (LSC) expansion that can serve as a potential alternative substrate to replace human amniotic membrane.

Methods:

The human Limbus stem cell was used to evaluate the biocompatibility of substrates (nanofibrous scaffold and, human amniotic membrane) based on their phenotypic profile, viability, proliferation, and attachment ability.

Results:

Biocompatibility results indicated that the all substrates were highly biocompatible, as LSCs could favorably attach and proliferate on the nanofibrous surface. Microscopic figures showed that the human limbal stem cells were firmly anchored to the substrates and were able to retain a normal corneal stem cell phenotype. Microscopic analyses illustrated that cells infiltrated the nanofibers and successfully formed a three-dimensional corneal epithelium, which was viable for two weeks. Immunocytochemistry (ICC) and real time–PCR results revealed no change in the expression profile of LECs grown on nanofibrous substrate when compared to those grown on human amniotic membrane.

Conclusion:

electrospun nanofibrous PHBV substrate provides not only a milieu supporting LSCs expansion, but also serve as a useful alternative carrier for ocular surface tissue engineering and could be used as an alternative substrate to amniotic membrane

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  - بـیــست و پنجمین کنــگــره سـالیـانه انـجـمـن چـشـم پـزشـکی ایـــران